Y‐BODY DETECTION IN FORMALIN‐FIXED LYMPH NODES, USING PROTEOLYTIC ENZYMES AND OXIDATIVE DEAMINATION

JØRGEN L. THOMSEN; IDA M. LISSE; NIELS H. HOLLAENDER

doi:10.1111/j.1699-0463.1987.tb00014_95A.x

Y‐BODY DETECTION IN FORMALIN‐FIXED LYMPH NODES, USING PROTEOLYTIC ENZYMES AND OXIDATIVE DEAMINATION

JØRGEN L. THOMSEN^*
, IDA M. LISSE
, NIELS H. HOLLAENDER

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

Abstract

The effect of proteolytic enzymes (Protease and Trypsin) and oxidative deamination (Chloramine‐T and Sodiumhypochlorite) on formalin‐fixed paraffin‐embedded lymph nodes with regard to detection of fluorescent Y‐bodies was examined. Protease was found to be more suitable than the others; acid hydrolysis was found to be unsuitable. The results of Y‐body detection were further improved by adding fixation of 30 minutes in Carnoy's solution.

Original language	English
Pages (from-to)	103-106
Number of pages	4
Journal	Acta Pathologica Microbiologica Scandinavica Series A :Pathology
Volume	95 A
Issue number	1-6
DOIs	https://doi.org/10.1111/j.1699-0463.1987.tb00014_95A.x
Publication status	Published - Jul 1987

Keywords

fluorescence
formalin fixation
lymph nodes
Y‐chromosomes

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title = "Y‐BODY DETECTION IN FORMALIN‐FIXED LYMPH NODES, USING PROTEOLYTIC ENZYMES AND OXIDATIVE DEAMINATION",

abstract = "The effect of proteolytic enzymes (Protease and Trypsin) and oxidative deamination (Chloramine‐T and Sodiumhypochlorite) on formalin‐fixed paraffin‐embedded lymph nodes with regard to detection of fluorescent Y‐bodies was examined. Protease was found to be more suitable than the others; acid hydrolysis was found to be unsuitable. The results of Y‐body detection were further improved by adding fixation of 30 minutes in Carnoy's solution.",

keywords = "fluorescence, formalin fixation, lymph nodes, Y‐chromosomes",

author = "THOMSEN, \{J{\O}RGEN L.\} and LISSE, \{IDA M.\} and HOLLAENDER, \{NIELS H.\}",

year = "1987",

month = jul,

doi = "10.1111/j.1699-0463.1987.tb00014\_95A.x",

language = "English",

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AU - LISSE, IDA M.

AU - HOLLAENDER, NIELS H.

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N2 - The effect of proteolytic enzymes (Protease and Trypsin) and oxidative deamination (Chloramine‐T and Sodiumhypochlorite) on formalin‐fixed paraffin‐embedded lymph nodes with regard to detection of fluorescent Y‐bodies was examined. Protease was found to be more suitable than the others; acid hydrolysis was found to be unsuitable. The results of Y‐body detection were further improved by adding fixation of 30 minutes in Carnoy's solution.

AB - The effect of proteolytic enzymes (Protease and Trypsin) and oxidative deamination (Chloramine‐T and Sodiumhypochlorite) on formalin‐fixed paraffin‐embedded lymph nodes with regard to detection of fluorescent Y‐bodies was examined. Protease was found to be more suitable than the others; acid hydrolysis was found to be unsuitable. The results of Y‐body detection were further improved by adding fixation of 30 minutes in Carnoy's solution.

KW - fluorescence

KW - formalin fixation

KW - lymph nodes

KW - Y‐chromosomes

UR - https://www.scopus.com/pages/publications/0023091058

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DO - 10.1111/j.1699-0463.1987.tb00014_95A.x

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JO - Acta Pathologica Microbiologica Scandinavica Series A :Pathology

JF - Acta Pathologica Microbiologica Scandinavica Series A :Pathology

IS - 1-6

ER -

Y‐BODY DETECTION IN FORMALIN‐FIXED LYMPH NODES, USING PROTEOLYTIC ENZYMES AND OXIDATIVE DEAMINATION

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