Abstract
A flow-through microsampling device that can be inserted in the column elution flow line of a chromatographic system has been developed. A sample drop (volume 2.2 nl) is ejected when a voltage pulse is applied to a piezo-electric transducer. The drops can be ejected with up to 70 Hz frequency, giving a sample flow range of 0-9 μl/min. Samples were collected on a moving agarose surface during elution of freshly prepared human serum. The precipitate pattern obtained after immunoelectrophoresis is similar to what would be obtained in a fused rocket electrophoresis of multiple samples from a fraction collector.
| Original language | English |
|---|---|
| Pages (from-to) | 181-190 |
| Number of pages | 10 |
| Journal | Journal of Biochemical and Biophysical Methods |
| Volume | 27 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Oct 1993 |
Keywords
- Flow-through liquid sampler
- Fused rocket electrophoresis
- Piezo-electric drop generator
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