A classical brown adipose tissue mrna signature partly overlaps with brite in the supraclavicular region of adult humans

Naja Zenius Jespersen; Therese Juhlin Larsen; Lone Peijs; Søren Daugaard; Preben Homøe; Annika Loft; Jasper De Jong; Neha Mathur; Barbara Cannon; Jan Nedergaard; Bente Klarlund Pedersen; Kirsten Møller; Camilla Scheele

doi:10.1016/j.cmet.2013.04.011

A classical brown adipose tissue mrna signature partly overlaps with brite in the supraclavicular region of adult humans

Naja Zenius Jespersen
, Therese Juhlin Larsen
, Lone Peijs
, Søren Daugaard
, Preben Homøe
, Annika Loft
, Jasper De Jong
, Neha Mathur
, Barbara Cannon
, Jan Nedergaard
, Bente Klarlund Pedersen
, Kirsten Møller
, Camilla Scheele^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Human brown adipose tissue (BAT) has been detected in adults but was recently suggested to be of brite/beige origin. We collected BAT from the supraclavicular region in 21 patients undergoing surgery for suspected cancer in the neck area and assessed the gene expression of established murine markers for brown, brite/beige, and white adipocytes. We demonstrate that a classical brown expression signature, including upregulation of miR-206, miR-133b, LHX8, and ZIC1 and downregulation of HOXC8 and HOXC9, coexists with an upregulation of two newly established brite/beige markers, TBX1 and TMEM26. A similar mRNA expression profile was observed when comparing isolated human adipocytes from BAT and white adipose tissue (WAT) depots, differentiated in vitro. In conclusion, our data suggest that human BAT might consist of both classical brown and recruitable brite adipocytes, an observation important for future considerations on how to induce human BAT.

Original language	English
Pages (from-to)	798-805
Number of pages	8
Journal	Cell Metabolism
Volume	17
Issue number	5
DOIs	https://doi.org/10.1016/j.cmet.2013.04.011
Publication status	Published - 7 May 2013
Externally published	Yes

Funding

The Centre of Inflammation and Metabolism (CIM) is supported by a grant from the Danish National Research Foundation (#02-512-55). This study was further supported by the Danish Council for Independent Research–Medical Sciences, The Danish Medical Society of Copenhagen, the Beckett Foundation, and the Augustinus Foundation. CIM is part of the UNIK Project: Food, Fitness & Pharma for Health and Disease, supported by the Danish Ministry of Science, Technology and Innovation. CIM is a member of DD2 - the Danish Center for Strategic Research in Type 2 Diabetes (the Danish Council for Strategic Research, grant no. 09-067009 and 09-075724). J.N. is on the Scientific Advisory Board of Ember Therapeutics and is also a shareholder. B.C. is on the Scientific and Medical Advisory Board of Metabolic Solutions Development Corporation.

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Jespersen, N. Z., Larsen, T. J., Peijs, L., Daugaard, S., Homøe, P., Loft, A., De Jong, J., Mathur, N., Cannon, B., Nedergaard, J., Pedersen, B. K., Møller, K., & Scheele, C. (2013). A classical brown adipose tissue mrna signature partly overlaps with brite in the supraclavicular region of adult humans. Cell Metabolism, 17(5), 798-805. https://doi.org/10.1016/j.cmet.2013.04.011

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title = "A classical brown adipose tissue mrna signature partly overlaps with brite in the supraclavicular region of adult humans",

abstract = "Human brown adipose tissue (BAT) has been detected in adults but was recently suggested to be of brite/beige origin. We collected BAT from the supraclavicular region in 21 patients undergoing surgery for suspected cancer in the neck area and assessed the gene expression of established murine markers for brown, brite/beige, and white adipocytes. We demonstrate that a classical brown expression signature, including upregulation of miR-206, miR-133b, LHX8, and ZIC1 and downregulation of HOXC8 and HOXC9, coexists with an upregulation of two newly established brite/beige markers, TBX1 and TMEM26. A similar mRNA expression profile was observed when comparing isolated human adipocytes from BAT and white adipose tissue (WAT) depots, differentiated in vitro. In conclusion, our data suggest that human BAT might consist of both classical brown and recruitable brite adipocytes, an observation important for future considerations on how to induce human BAT.",

author = "Jespersen, \{Naja Zenius\} and Larsen, \{Therese Juhlin\} and Lone Peijs and S{\o}ren Daugaard and Preben Hom{\o}e and Annika Loft and \{De Jong\}, Jasper and Neha Mathur and Barbara Cannon and Jan Nedergaard and Pedersen, \{Bente Klarlund\} and Kirsten M{\o}ller and Camilla Scheele",

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doi = "10.1016/j.cmet.2013.04.011",

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Jespersen, NZ, Larsen, TJ, Peijs, L, Daugaard, S, Homøe, P, Loft, A, De Jong, J, Mathur, N, Cannon, B, Nedergaard, J, Pedersen, BK, Møller, K & Scheele, C 2013, 'A classical brown adipose tissue mrna signature partly overlaps with brite in the supraclavicular region of adult humans', Cell Metabolism, vol. 17, no. 5, pp. 798-805. https://doi.org/10.1016/j.cmet.2013.04.011

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T1 - A classical brown adipose tissue mrna signature partly overlaps with brite in the supraclavicular region of adult humans

AU - Jespersen, Naja Zenius

AU - Larsen, Therese Juhlin

AU - Peijs, Lone

AU - Daugaard, Søren

AU - Homøe, Preben

AU - Loft, Annika

AU - De Jong, Jasper

AU - Mathur, Neha

AU - Cannon, Barbara

AU - Nedergaard, Jan

AU - Pedersen, Bente Klarlund

AU - Møller, Kirsten

AU - Scheele, Camilla

PY - 2013/5/7

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AB - Human brown adipose tissue (BAT) has been detected in adults but was recently suggested to be of brite/beige origin. We collected BAT from the supraclavicular region in 21 patients undergoing surgery for suspected cancer in the neck area and assessed the gene expression of established murine markers for brown, brite/beige, and white adipocytes. We demonstrate that a classical brown expression signature, including upregulation of miR-206, miR-133b, LHX8, and ZIC1 and downregulation of HOXC8 and HOXC9, coexists with an upregulation of two newly established brite/beige markers, TBX1 and TMEM26. A similar mRNA expression profile was observed when comparing isolated human adipocytes from BAT and white adipose tissue (WAT) depots, differentiated in vitro. In conclusion, our data suggest that human BAT might consist of both classical brown and recruitable brite adipocytes, an observation important for future considerations on how to induce human BAT.

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A classical brown adipose tissue mrna signature partly overlaps with brite in the supraclavicular region of adult humans

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