Y‐BODY DETECTION IN FORMALIN‐FIXED LYMPH NODES, USING PROTEOLYTIC ENZYMES AND OXIDATIVE DEAMINATION

JØRGEN L. THOMSEN; IDA M. LISSE; NIELS H. HOLLAENDER

doi:10.1111/j.1699-0463.1987.tb00014_95A.x

Y‐BODY DETECTION IN FORMALIN‐FIXED LYMPH NODES, USING PROTEOLYTIC ENZYMES AND OXIDATIVE DEAMINATION

JØRGEN L. THOMSEN^*, IDA M. LISSE, NIELS H. HOLLAENDER

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift › Artikel › Forskning › peer review

Abstract

The effect of proteolytic enzymes (Protease and Trypsin) and oxidative deamination (Chloramine‐T and Sodiumhypochlorite) on formalin‐fixed paraffin‐embedded lymph nodes with regard to detection of fluorescent Y‐bodies was examined. Protease was found to be more suitable than the others; acid hydrolysis was found to be unsuitable. The results of Y‐body detection were further improved by adding fixation of 30 minutes in Carnoy's solution.

Originalsprog	Engelsk
Sider (fra-til)	103-106
Antal sider	4
Tidsskrift	Acta Pathologica Microbiologica Scandinavica Series A :Pathology
Vol/bind	95 A
Udgave nummer	1-6
DOI	https://doi.org/10.1111/j.1699-0463.1987.tb00014_95A.x
Status	Udgivet - jul. 1987

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10.1111/j.1699-0463.1987.tb00014_95A.x

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title = "Y‐BODY DETECTION IN FORMALIN‐FIXED LYMPH NODES, USING PROTEOLYTIC ENZYMES AND OXIDATIVE DEAMINATION",

abstract = "The effect of proteolytic enzymes (Protease and Trypsin) and oxidative deamination (Chloramine‐T and Sodiumhypochlorite) on formalin‐fixed paraffin‐embedded lymph nodes with regard to detection of fluorescent Y‐bodies was examined. Protease was found to be more suitable than the others; acid hydrolysis was found to be unsuitable. The results of Y‐body detection were further improved by adding fixation of 30 minutes in Carnoy's solution.",

keywords = "fluorescence, formalin fixation, lymph nodes, Y‐chromosomes",

author = "THOMSEN, {J{\O}RGEN L.} and LISSE, {IDA M.} and HOLLAENDER, {NIELS H.}",

year = "1987",

month = jul,

doi = "10.1111/j.1699-0463.1987.tb00014_95A.x",

language = "English",

volume = "95 A",

pages = "103--106",

journal = "Acta Pathologica Microbiologica Scandinavica Series A :Pathology",

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AU - THOMSEN, JØRGEN L.

AU - LISSE, IDA M.

AU - HOLLAENDER, NIELS H.

PY - 1987/7

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N2 - The effect of proteolytic enzymes (Protease and Trypsin) and oxidative deamination (Chloramine‐T and Sodiumhypochlorite) on formalin‐fixed paraffin‐embedded lymph nodes with regard to detection of fluorescent Y‐bodies was examined. Protease was found to be more suitable than the others; acid hydrolysis was found to be unsuitable. The results of Y‐body detection were further improved by adding fixation of 30 minutes in Carnoy's solution.

AB - The effect of proteolytic enzymes (Protease and Trypsin) and oxidative deamination (Chloramine‐T and Sodiumhypochlorite) on formalin‐fixed paraffin‐embedded lymph nodes with regard to detection of fluorescent Y‐bodies was examined. Protease was found to be more suitable than the others; acid hydrolysis was found to be unsuitable. The results of Y‐body detection were further improved by adding fixation of 30 minutes in Carnoy's solution.

KW - fluorescence

KW - formalin fixation

KW - lymph nodes

KW - Y‐chromosomes

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U2 - 10.1111/j.1699-0463.1987.tb00014_95A.x

DO - 10.1111/j.1699-0463.1987.tb00014_95A.x

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SN - 0108-0164

VL - 95 A

SP - 103

EP - 106

JO - Acta Pathologica Microbiologica Scandinavica Series A :Pathology

JF - Acta Pathologica Microbiologica Scandinavica Series A :Pathology

IS - 1-6

ER -

Y‐BODY DETECTION IN FORMALIN‐FIXED LYMPH NODES, USING PROTEOLYTIC ENZYMES AND OXIDATIVE DEAMINATION

Abstract

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