TY - JOUR
T1 - Vaccination for birch pollen allergy
T2 - Comparison of the affinities of specific immunoglobulins E, G1 and G4 measured by surface plasmon resonance
AU - Jakobsen, Charlotte Georgi
AU - Bodtger, U.
AU - Poulsen, L. K.
AU - Roggen, E. L.
PY - 2005/2/1
Y1 - 2005/2/1
N2 - Background: Allergen-specific immunotherapy (SIT) is associated with increased levels of allergen-specific IgG in serum. However, it is not clear to what extent qualitative changes in the allergen binding capacity of IgG may be induced as well. Objective: The purpose of this study was to investigate the influences of SIT on antibody affinity. Methods: The binding affinity of purified serum IgG1, IgG4 and IgE to the major allergen in birch (Betula verrucosa) pollen, Bet v 1, was analysed by surface plasmon resonance. The antibodies were obtained from 10 birch pollen-allergic patients receiving SIT and from 10 patients with no SIT. Results: The patients having received SIT have a significant higher titre of anti-Bet v 1 antibodies in their blood, but the affinity to Bet v 1 of allergen-specific IgE, IgG 1 and IgG4 does not differ between the two groups. For IgG1 and IgG4, correlations between less allergic symptoms and affinity of the antibodies were observed both in the SIT group and to a smaller extent in the non-SIT group. Conclusion: SIT has no effect on antibody affinity of allergen-specific IgE, IgG1 or IgG4. Allergic patients with high-affinity IgG1 and IgG4 antibodies report less symptoms than patients with low-affinity antibodies.
AB - Background: Allergen-specific immunotherapy (SIT) is associated with increased levels of allergen-specific IgG in serum. However, it is not clear to what extent qualitative changes in the allergen binding capacity of IgG may be induced as well. Objective: The purpose of this study was to investigate the influences of SIT on antibody affinity. Methods: The binding affinity of purified serum IgG1, IgG4 and IgE to the major allergen in birch (Betula verrucosa) pollen, Bet v 1, was analysed by surface plasmon resonance. The antibodies were obtained from 10 birch pollen-allergic patients receiving SIT and from 10 patients with no SIT. Results: The patients having received SIT have a significant higher titre of anti-Bet v 1 antibodies in their blood, but the affinity to Bet v 1 of allergen-specific IgE, IgG 1 and IgG4 does not differ between the two groups. For IgG1 and IgG4, correlations between less allergic symptoms and affinity of the antibodies were observed both in the SIT group and to a smaller extent in the non-SIT group. Conclusion: SIT has no effect on antibody affinity of allergen-specific IgE, IgG1 or IgG4. Allergic patients with high-affinity IgG1 and IgG4 antibodies report less symptoms than patients with low-affinity antibodies.
KW - Affinity
KW - Allergen specific immunotherapy
KW - Allergy
KW - Bet v 1
KW - IgE
KW - IgG1
KW - IgG4
KW - Surface plasmon resonance
UR - http://www.scopus.com/inward/record.url?scp=14844351310&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2222.2005.02160.x
DO - 10.1111/j.1365-2222.2005.02160.x
M3 - Article
C2 - 15725191
AN - SCOPUS:14844351310
SN - 0954-7894
VL - 35
SP - 193
EP - 198
JO - Clinical and Experimental Allergy
JF - Clinical and Experimental Allergy
IS - 2
ER -