Urinary proteomics in diabetes and CKD

Kasper Rossing*, Harald Mischak, Mohammed Dakna, Petra Zürbig, Jan Novak, Bruce A. Julian, David M. Good, Joshua J. Coon, Lise Tarnow, Peter Rossing

*Corresponding author af dette arbejde

    Publikation: Bidrag til tidsskriftArtikelForskningpeer review

    Abstract

    Urinary biomarkers for diabetes, diabetic nephropathy, and nondiabetic proteinuric renal diseases were sought. For 305 individuals, biomarkers were defined and validated in blinded data sets using high-resolution capillary electrophoresis coupled with electrospray-ionization mass spectrometry. A panel of 40 biomarkers distinguished patients with diabetes from healthy individuals with 89% sensitivity and 91% specificity. Among patients with diabetes, 102 urinary biomarkers differed significantly between patients with normoalbuminuria and nephropathy, and a model that included 65 of these correctly identified diabetic nephropathy with 97% sensitivity and specificity. Furthermore, this panel of biomarkers identified patients who had microalbuminuria and diabetes and progressed toward overt diabetic nephropathy over 3 yr. Differentiation between diabetic nephropathy and other chronic renal diseases reached 81% sensitivity and 91% specificity. Many of the biomarkers were fragments of collagen type I, and quantities were reduced in patients with diabetes or diabetic nephropathy. In conclusion, this study shows that analysis of the urinary proteome may allow early detection of diabetic nephropathy and may provide prognostic information.

    OriginalsprogEngelsk
    Sider (fra-til)1283-1290
    Antal sider8
    TidsskriftJournal of the American Society of Nephrology
    Vol/bind19
    Udgave nummer7
    DOI
    StatusUdgivet - 1 jul. 2008

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