The impact of inter-laboratory glucose bias on the diagnosis of gestational diabetes mellitus: Comparison of common automated central laboratory methods

Cathrine Munk Scheuer*, Casper Duevang Tvarnø, Charlotte Gils, Julie Dahl Ravn, H David McIntyre, Dorte Møller Jensen, Peter Damm, Jeannet Kepp Bruun Lauenborg, Tine Dalsgaard Clausen, Martin Overgaard

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskriftArtikelForskningpeer review

Abstract

BACKGROUND AND AIMS: The diagnosis of gestational diabetes mellitus (GDM) is based exclusively on glucose measurements, which are highly influenced by pre-analytical and analytical factors. Therefore, poor agreement across laboratories may affect the prevalence of GDM. We aimed to determine the inter-laboratory bias of glucose measurements and the impact on GDM prevalence.

MATERIAL AND METHODS: A prospective cohort study of women (n = 110) referred for second-trimester GDM diagnostics using a 75 g oral glucose tolerance test. Maternal glucose was assessed from venous plasma at fasting, 1 h and 2 h. Venous blood were collected in Fluoride Citrate tubes and frozen. Samples were analyzed at five central laboratories using four different automated glucose Hexokinase methods and GDM prevalence was evaluated according to WHO2013 diagnostic criteria.

RESULTS: Maximum inter-laboratory bias was 0.19, 0.30 and 0.27 mmol/L in fasting, 1 h and 2 h samples, respectively. GDM prevalence ranged 30.0-41.1% across laboratories.

CONCLUSION: Inter-laboratory bias for mean venous glucose was low and within desirable limits. Nonetheless, the impact on GDM prevalence was considerable, which may inappropriately affect clinical practice.

OriginalsprogEngelsk
Artikelnummer117414
TidsskriftClinica Chimica Acta
Vol/bind546
Tidlig onlinedato30 maj 2023
DOI
StatusUdgivet - 1 jun. 2023

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Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.

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