Quantification of the maximum capacity for active sodium‐potassium transport in rat skeletal muscle.

T. Clausen*, M. E. Everts, K. Kjeldsen

*Corresponding author af dette arbejde

    Publikation: Bidrag til tidsskriftArtikelForskningpeer review

    Abstract

    1. Intact skeletal muscle fibres have been shown to contain a high concentration of [3H]ouabain binding sites (100‐800 pmol g wet wt.‐1). Under resting conditions, however, it seems that in isolated muscles only 2‐6% of the corresponding expected capacity for active Na+‐K+ transport is utilized. 2. In order to determine whether all [3H]ouabain binding sites in rat soleus muscle represent functional Na+‐K+ pumps, we have measured the maximum rates of the ouabain‐suppressible components of isotopic fluxes of Na+ and K+ as well as the net changes in Na+‐K+ contents. 3. Experiments with soleus muscles isolated from 4‐week‐old rats showed that following Na+ loading (I.C. Na+, 126 mmol l‐1), the ouabain‐suppressible 86Rb+ uptake and 22Na+ efflux as measured during 3 min of exposure to K+‐rich buffer were 5800 and 6500 nmol g wet wt.‐1 min‐1, respectively. 4. These initial high rates of isotopic fluxes were confirmed by flame photometric measurements of Na+‐K+ contents. The ouabain‐suppressible 86Rb+ uptake had a temperature coefficient of 2.1, was inhibited by 2,4‐dinitrophenol, but showed no response to tetracaine, BaCl2, Ca2+‐free buffer or tetraethylammonium chloride. 5. In soleus muscles, where the total population of [3H]ouabain binding sites had undergone changes as a result of differentiation, K+ depletion or pre‐treatment with thyroid hormone, there was a significant correlation (r = 0.95, P less than 0.005) between the concentration of [3H]ouabain binding sites (260‐1170 pmol g wet wt.‐1) and the maximum ouabain‐suppressible 86Rb+ uptake (2300‐10,900 nmol g wet wt.‐1 min‐1). 6. It is concluded that by the combination of Na+ loading and high extracellular K+, the available Na+‐K+ pumps as quantified by the [3H]ouabain binding capacity can be activated to reach a transport rate around 90% of the theoretical maximum at 30 degrees C.

    OriginalsprogEngelsk
    Sider (fra-til)163-181
    Antal sider19
    TidsskriftThe Journal of Physiology
    Vol/bind388
    Udgave nummer1
    DOI
    StatusUdgivet - 1 jul. 1987

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