TY - JOUR
T1 - Quality assessment of a placental perfusion protocol
AU - Mathiesen, Line
AU - Mose, Tina
AU - Mørck, Thit Juul
AU - Nielsen, Jeanette Kolstrup Søgaard
AU - Nielsen, Leif Kofoed
AU - Maroun, Lisa Leth
AU - Dziegiel, Morten Hanefeld
AU - Larsen, Lise Grupe
AU - Knudsen, Lisbeth Ehlert
PY - 2010/8/1
Y1 - 2010/8/1
N2 - Validation of in vitro test systems using the modular approach with steps addressing reliability and relevance is an important aim when developing in vitro tests in e.g. reproductive toxicology. The ex vivo human placental perfusion system may be used for such validation, here presenting the placental perfusion model in Copenhagen including control substances. The positive control substance antipyrine shows no difference in transport regardless of perfusion media used or of terms of delivery (n=59, p<0.05). Negative control studies with FITC marked dextran correspond with leakage criteria (<3mlh-1 from the fetal reservoir) when adding 2 (n=7) and 20mg (n=9) FITC-dextran/100ml fetal perfusion media. Success rate of the Copenhagen placental perfusions is provided in this study, including considerations and quality control parameters. Three checkpoints suggested to determine success rate revealed that 15% of the cannulated placentae received in one year (n=202) were successfully perfused.
AB - Validation of in vitro test systems using the modular approach with steps addressing reliability and relevance is an important aim when developing in vitro tests in e.g. reproductive toxicology. The ex vivo human placental perfusion system may be used for such validation, here presenting the placental perfusion model in Copenhagen including control substances. The positive control substance antipyrine shows no difference in transport regardless of perfusion media used or of terms of delivery (n=59, p<0.05). Negative control studies with FITC marked dextran correspond with leakage criteria (<3mlh-1 from the fetal reservoir) when adding 2 (n=7) and 20mg (n=9) FITC-dextran/100ml fetal perfusion media. Success rate of the Copenhagen placental perfusions is provided in this study, including considerations and quality control parameters. Three checkpoints suggested to determine success rate revealed that 15% of the cannulated placentae received in one year (n=202) were successfully perfused.
KW - Antipyrine
KW - Caesarean section
KW - FITC-dextran
KW - Modular validation approach
KW - Placental perfusion transport model
KW - Success rate
KW - Vaginal birth
UR - http://www.scopus.com/inward/record.url?scp=77954089461&partnerID=8YFLogxK
U2 - 10.1016/j.reprotox.2010.01.006
DO - 10.1016/j.reprotox.2010.01.006
M3 - Article
C2 - 20096346
AN - SCOPUS:77954089461
SN - 0890-6238
VL - 30
SP - 138
EP - 146
JO - Reproductive Toxicology
JF - Reproductive Toxicology
IS - 1
ER -