Neuronal pH regulation: Constant normal intracellular pH is maintained in brain during low extracellular pH induced by acetazolamide-31P NMR study

S. Vorstrup, K. E. Jensen, C. Thomsen, O. Henriksen, N. A. Lassen, O. B. Paulson

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    Abstrakt

    The intracellular pH in the brain was studied in six healthy volunteers before and immediately after the administration of 2 g of acetazolamide. Phosphorus-31 nuclear magnetic resonance spectroscopy by a 1.5 tesla whole-body scanner was used. The chemical shift between the inorganic phosphate and the phosphocreatine resonance frequencies was used for indirect assessment of the intracellular pH. The mean baseline intracellular pH was 7.05 ± 0.04 (SD). The mean pH changes obtained at 15-min intervals within the first hour of acetazolamide administration were -0.03 ± 0.04 (SD), -0.02 ± 0.03 (SD), and 0.00 ± 0.04 (SD), i.e., no statistically significant pH decrease was observed during the period where extracellular pH is known to drop markedly. Although several factors contribute to the lack of change of the intraneuronal pH, we will discuss that this observation in addition might suggest a direct intracerebral effect of acetazolamide.

    OriginalsprogEngelsk
    Sider (fra-til)417-421
    Antal sider5
    TidsskriftJournal of Cerebral Blood Flow and Metabolism
    Vol/bind9
    Udgave nummer3
    DOI
    StatusUdgivet - 1 jan. 1989

    Fingeraftryk Udforsk hvilke forskningsemner 'Neuronal pH regulation: Constant normal intracellular pH is maintained in brain during low extracellular pH induced by acetazolamide-<sup>31</sup>P NMR study' indeholder.

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