Measurement of plasma viscosity by free oscillation rheometry: imprecision, sample stability and establishment of a new reference range

Lise Pedersen, Erling B Nielsen, Majbrit K Christensen, Maybritt Buchwald, Mads Nybo

    Publikation: Bidrag til tidsskriftArtikelForskningpeer review

    Abstrakt

    BACKGROUND: Plasma viscosity (PV) is used in the investigation of hyperviscosity syndrome. We have evaluated the performance of a new bench-top free oscillation rheometer, ReoRox from Medirox, and established a new reference range.

    METHODS: Performance of the free oscillation rheometry (FOR) method was evaluated examining within-run and between-run imprecision, duration of measurement and operator simplicity. Furthermore, influence of storage on the PV measurements was assessed and a reference range based on plasma from 585 healthy men and women was established.

    RESULTS: Imprecision of the free oscillation rheometer was comparable to other viscometers and performance of the instrument was satisfying. The non-parametrical reference range established was 1.22-1.44 mPa s at 37℃ (2.5th and 97.5th percentiles).

    CONCLUSIONS: We have established a FOR PV reference range to support clinical decision-making. Furthermore, we found high pre-analytical stability of the samples and an analytical imprecision comparable to other PV methods.

    OriginalsprogEngelsk
    Sider (fra-til)495-8
    Antal sider4
    TidsskriftAnnals of Clinical Biochemistry
    Vol/bind51
    Udgave nummerPt 4
    DOI
    StatusUdgivet - jul. 2014

    Fingeraftryk Udforsk hvilke forskningsemner 'Measurement of plasma viscosity by free oscillation rheometry: imprecision, sample stability and establishment of a new reference range' indeholder.

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