Measurement of plasma viscosity by free oscillation rheometry: imprecision, sample stability and establishment of a new reference range

Lise Pedersen; Erling B Nielsen; Majbrit K Christensen; Maybritt Buchwald; Mads Nybo

doi:10.1177/0004563213504550

Measurement of plasma viscosity by free oscillation rheometry: imprecision, sample stability and establishment of a new reference range

Lise Pedersen, Erling B Nielsen, Majbrit K Christensen, Maybritt Buchwald, Mads Nybo

Publikation: Bidrag til tidsskrift › Artikel › Forskning › peer review

Abstrakt

BACKGROUND: Plasma viscosity (PV) is used in the investigation of hyperviscosity syndrome. We have evaluated the performance of a new bench-top free oscillation rheometer, ReoRox from Medirox, and established a new reference range.

METHODS: Performance of the free oscillation rheometry (FOR) method was evaluated examining within-run and between-run imprecision, duration of measurement and operator simplicity. Furthermore, influence of storage on the PV measurements was assessed and a reference range based on plasma from 585 healthy men and women was established.

RESULTS: Imprecision of the free oscillation rheometer was comparable to other viscometers and performance of the instrument was satisfying. The non-parametrical reference range established was 1.22-1.44 mPa s at 37℃ (2.5th and 97.5th percentiles).

CONCLUSIONS: We have established a FOR PV reference range to support clinical decision-making. Furthermore, we found high pre-analytical stability of the samples and an analytical imprecision comparable to other PV methods.

Originalsprog	Engelsk
Sider (fra-til)	495-8
Antal sider	4
Tidsskrift	Annals of Clinical Biochemistry
Vol/bind	51
Udgave nummer	Pt 4
DOI	https://doi.org/10.1177/0004563213504550
Status	Udgivet - jul. 2014

Adgang til dokumentet

10.1177/0004563213504550

Fingeraftryk Udforsk hvilke forskningsemner 'Measurement of plasma viscosity by free oscillation rheometry: imprecision, sample stability and establishment of a new reference range' indeholder.

Citationsformater

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abstract = "BACKGROUND: Plasma viscosity (PV) is used in the investigation of hyperviscosity syndrome. We have evaluated the performance of a new bench-top free oscillation rheometer, ReoRox from Medirox, and established a new reference range.METHODS: Performance of the free oscillation rheometry (FOR) method was evaluated examining within-run and between-run imprecision, duration of measurement and operator simplicity. Furthermore, influence of storage on the PV measurements was assessed and a reference range based on plasma from 585 healthy men and women was established.RESULTS: Imprecision of the free oscillation rheometer was comparable to other viscometers and performance of the instrument was satisfying. The non-parametrical reference range established was 1.22-1.44 mPa s at 37℃ (2.5th and 97.5th percentiles).CONCLUSIONS: We have established a FOR PV reference range to support clinical decision-making. Furthermore, we found high pre-analytical stability of the samples and an analytical imprecision comparable to other PV methods.",

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author = "Lise Pedersen and Nielsen, {Erling B} and Christensen, {Majbrit K} and Maybritt Buchwald and Mads Nybo",

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Measurement of plasma viscosity by free oscillation rheometry : imprecision, sample stability and establishment of a new reference range. / Pedersen, Lise; Nielsen, Erling B; Christensen, Majbrit K; Buchwald, Maybritt; Nybo, Mads.

I: Annals of Clinical Biochemistry, Bind 51, Nr. Pt 4, 07.2014, s. 495-8.

Publikation: Bidrag til tidsskrift › Artikel › Forskning › peer review

TY - JOUR

T1 - Measurement of plasma viscosity by free oscillation rheometry

T2 - imprecision, sample stability and establishment of a new reference range

AU - Pedersen, Lise

AU - Nielsen, Erling B

AU - Christensen, Majbrit K

AU - Buchwald, Maybritt

AU - Nybo, Mads

N1 - © The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

PY - 2014/7

Y1 - 2014/7

N2 - BACKGROUND: Plasma viscosity (PV) is used in the investigation of hyperviscosity syndrome. We have evaluated the performance of a new bench-top free oscillation rheometer, ReoRox from Medirox, and established a new reference range.METHODS: Performance of the free oscillation rheometry (FOR) method was evaluated examining within-run and between-run imprecision, duration of measurement and operator simplicity. Furthermore, influence of storage on the PV measurements was assessed and a reference range based on plasma from 585 healthy men and women was established.RESULTS: Imprecision of the free oscillation rheometer was comparable to other viscometers and performance of the instrument was satisfying. The non-parametrical reference range established was 1.22-1.44 mPa s at 37℃ (2.5th and 97.5th percentiles).CONCLUSIONS: We have established a FOR PV reference range to support clinical decision-making. Furthermore, we found high pre-analytical stability of the samples and an analytical imprecision comparable to other PV methods.

AB - BACKGROUND: Plasma viscosity (PV) is used in the investigation of hyperviscosity syndrome. We have evaluated the performance of a new bench-top free oscillation rheometer, ReoRox from Medirox, and established a new reference range.METHODS: Performance of the free oscillation rheometry (FOR) method was evaluated examining within-run and between-run imprecision, duration of measurement and operator simplicity. Furthermore, influence of storage on the PV measurements was assessed and a reference range based on plasma from 585 healthy men and women was established.RESULTS: Imprecision of the free oscillation rheometer was comparable to other viscometers and performance of the instrument was satisfying. The non-parametrical reference range established was 1.22-1.44 mPa s at 37℃ (2.5th and 97.5th percentiles).CONCLUSIONS: We have established a FOR PV reference range to support clinical decision-making. Furthermore, we found high pre-analytical stability of the samples and an analytical imprecision comparable to other PV methods.

KW - Adolescent

KW - Adult

KW - Aged

KW - Blood Viscosity

KW - Female

KW - Humans

KW - Leukemia/blood

KW - Male

KW - Middle Aged

KW - Multiple Myeloma/blood

KW - Rheology/instrumentation

KW - Syndrome

KW - Waldenstrom Macroglobulinemia/blood

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