Interleukins increase surface ganglioside expression of pancreatic islet cells in vitro

T. W. KJAER*, J. RYGAARD, K. BENDTZEN, K. JOSEFSEN, T. BOCK, K. BUSCHARD

*Corresponding author af dette arbejde

    Publikation: Bidrag til tidsskriftArtikelForskningpeer review

    Abstract

    This experiment was conducted in order to investigate whether expression of gangliosides on islet cell surface in vitro is influenced by cytokines, especially interleukin 1. Islets from adult Lewis rats were incubated with different concentrations of recombinant‐derived human cytokines. Following dispase treatment, the single cells were labeled with monoclonal antiganglioside antibodies A2B5 or R2D6, and conjugate. Both are directed against β cells; A2B5 is recognized to bind specifically to pancreatic islet cells, while R2D6 is shown to bind no other pancreatic cells than β cells. Surface labeling was evaluated in blind trials using a fluorescence microscope and a fluorescence‐activated cell sorter (FACS). A2B5 staining demonstrated a significantly higher number of labeled cells after incubation with interleukin la (14.9%±2.8; p<0.005), interleukin 1β (23.2%±4.2; p<0.0005) or TNFα (16.1%± 4.0; p = 0.005) compared to endotoxin controls (4.1%±1.1). Interleukin 1β (9.5%β1.5; p<0.005) showed a significantly increased number of R2D6‐stained cells (control: 2.3%±1.3). A similar but not significant effect was seen with interleukin 1α and TNFα. Interleukin 6 had no effect on the antigen expression. The intensity of labeling was elevated among interleukin 1β‐incubated cells compared to control samples. Thus, treatment of islets with different cytokines, especially interleukin 1β, increases surface antigen expression. We suggest that this mechanism of action in vitro may be of importance for the putative diabetogenic effect of interleukin 1.

    OriginalsprogEngelsk
    Sider (fra-til)509-514
    Antal sider6
    TidsskriftAPMIS
    Vol/bind100
    Udgave nummer1-6
    DOI
    StatusUdgivet - jan. 1992

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