Inter-observer agreement of Tumor Infiltrating Lymphocytes in primary HER2-positive breast cancer and correlation between Tissue microarray and full tumor-sections

Frances Mary Reznitsky*, Jeanette Dupont Jensen, Ann Knoop, Anne-Vibeke Laenkholm

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskriftArtikelForskningpeer review

Abstract

Tumor infiltrating lymphocytes (TILs) have predictive and prognostic potential in HER2-positive breast cancer (HER2+BC). Due to tumor heterogeneity, guidelines recommend evaluation on full tumor-sections over biopsies, but aren't clear regarding tissue microarrays (TMAs). Herein, we investigate the inter-observer agreement of TILs assessment in HER2+BC on full-sections and TMAs using a standardized method. Two pathologists assessed TILs using HE full-sections and TMAs from 244 patients with HER2+BC. TILs were assessed in 10% intervals; values <10% evaluated as 0%, 1% or 5%. Levels of agreement were evaluated using intraclass-coefficient (ICC), Kappa statistics and concordance analysis. For inter-observer agreement for full-sections, ICC was 0.93 (95% CI 0.89-0.95) and Kappa was 0.75, corresponding to acceptable and moderate agreement respectively. For TMAs, ICC was 0.73 (95% CI: 0.62-0.81) and Kappa 0.33, corresponding to unacceptable agreement. For association in matched TMA and full-sections, ICC was 0.64 (95% CI 0.55-0.71) and Lin's concordance correlation coefficient was 0.63 (95% CI 0.55-0.71), corresponding to unacceptable agreement. There is acceptable inter-observer agreement of TILs assessment on full-sections but not TMAs and discrepancy between full-sections and TMAs. TMA preparation must include consideration for representation of both entire tumor area and tumor-microenvironment to correctly define prognostic and predictive values of potential immuno-related biomarkers.

OriginalsprogEngelsk
Sider (fra-til)545-550
Antal sider6
TidsskriftActa pathologica et microbiologica Scandinavica
Vol/bind130
Udgave nummer9
Tidlig onlinedato31 maj 2022
DOI
StatusUdgivet - sep. 2022

Bibliografisk note

This article is protected by copyright. All rights reserved.

© 2022 APMIS. Published by John Wiley & Sons Ltd.

Fingeraftryk

Udforsk hvilke forskningsemner 'Inter-observer agreement of Tumor Infiltrating Lymphocytes in primary HER2-positive breast cancer and correlation between Tissue microarray and full tumor-sections' indeholder.

Citationsformater