Increased numbers of circulating basophils with decreased releasability after administration of rhG-CSF to allergic patients

M. Pedersen, K. S. Kristensen, P. Clementsen, O. T. Olsen, P. S. Skov, H. Permin, S. Norn

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    Abstrakt

    Preliminary studies in hematological patients have indicated that treatment with rhG-CSF reduces basophil releasability ex vivo. We examined this phenomenon further, in allergic patients. Ten patients with grass pollen rhinoconjunctivitis were given rhG-CSF (5 μg/kg/day s.c.) for 5 days, and examined before and after treatment. Basophil counts increased from 5 to 19×109/l (P<0.01). Total blood histamine increased from 80 to 160 μg/l (P<0.01), corresponding to a decrease in average basophil histamine content from 1.5 to 0.81 pg/cell (P<0.01). Isolated mononuclear cells showed a significantly decreased histamine release (HR) when stimulated with A23187 and grass. Whole blood experiments showed a similar decreased HR to grass and anti-IgE (P<0.01). However, we found an increase in total blood histamine. We conclude that treatment with rhG-CSF (1) increases the number of circulating blood basophils, (2) reduces the average histamine content per basophil, and (3) reduces the basophil releasability. These findings could be due to the mobilization of immature basophils from the bone marrow.

    OriginalsprogEngelsk
    Sider (fra-til)C24-C25
    TidsskriftAgents and Actions
    Vol/bind41
    Udgave nummer1 Supplement
    DOI
    StatusUdgivet - 1 jun. 1994

    Fingeraftryk Udforsk hvilke forskningsemner 'Increased numbers of circulating basophils with decreased releasability after administration of rhG-CSF to allergic patients' indeholder.

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