In vitro labeling of human polymorphonuclear leukocytes with 99mTc

Henning Kelbaek, Jan Fogh, Jens Elmgreen

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    Abstrakt

    Preliminary experience with 99mTc labeling of isolated polymorphonuclear leukocytes is reported. Leukocytes efficiently purified from 100 ml peripheral blood were labeled in vitro with 99mTc after pretreatment with small amounts of a stannous agent to reduce pertechnetate. A labeling yield of 1.3 mCi equivalent to 12.5% of the added dose of 99mTc and a cell binding efficiency of more than 90% was achieved under sterile conditions. Viability assays showed no influence of the labeling procedure on cell function. The technique may prove a valuable alternative for assessment of local leukocyte accumulation by scintigraphy.

    OriginalsprogEngelsk
    Sider (fra-til)366-369
    Antal sider4
    TidsskriftEuropean Journal of Nuclear Medicine
    Vol/bind9
    Udgave nummer8
    DOI
    StatusUdgivet - 1 aug. 1984

    Fingeraftryk Udforsk hvilke forskningsemner 'In vitro labeling of human polymorphonuclear leukocytes with <sup>99m</sup>Tc' indeholder.

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