Identification of patient-specific peptides for detection of M-proteins and myeloma cells

Pal B. Szecsi*, Erik Riise, Lisbeth B. Roslund, Jan Engberg, Ingemar Turesson, Lone Bunl, Claus Scuafer

*Corresponding author af dette arbejde

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    Abstrakt

    We have taken advantage of the selection power of phage display technology to define specific peptide mimotopes that recognize individual M- proteins, isolated from patients with multiple myeloma. Preferred amino acid motifs of phages binding to M-proteins were identified in 6/9 patients investigated. Chemically synthesized peptides, corresponding to the phage- displayed peptide inserts, were used to verify the specificity of binding in competition assays. The peptides were able to bind to the M-proteins, as well as the myeloma cells, with high sensitivity and specificity. Employing simple immunological techniques, < 0.01 g/l of M-protein could be quantified, suggesting a novel way for monitoring minimal residual disease in the production of guidelines for adjusting or reintroducing conventional chemotherapy. The peptide mimotopes defined by this technology may be useful as tumour-specific targeting agents and as a tool for purging cells in autologous bone marrow transplantation.

    OriginalsprogEngelsk
    Sider (fra-til)357-364
    Antal sider8
    TidsskriftBritish Journal of Haematology
    Vol/bind107
    Udgave nummer2
    DOI
    StatusUdgivet - 22 nov. 1999

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