TY - JOUR
T1 - Human and rodent muscle Na+-K+-ATPase in diabetes related to insulin, starvation, and training
AU - Schmidt, T. A.
AU - Hasselbalch, S.
AU - Farrell, P. A.
AU - Vestergaard, H.
AU - Kjeldsen, K.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - As determined by vanadate-facilitated [3H]ouabain binding to intact samples, semistarvation and untreated streptozotocin- or partial pancreatectomy-induced diabetes reduced rat soleus muscle Na+-K+- adenosinetriphosphatase (Na+-K+-ATPase) concentration by 12-21% (P < 0.05). Conversely, insulin treatment of rats with streptozotocin-induced diabetes induced an increase of 18-26% above control (P < 0.05). Treadmill training diminished the reduction in muscle [3H]ouabain binding site concentration induced by untreated diabetes to only 2-5%. No significant variation was observed in rat cerebral cortex Na+-K+-ATPase concentration as a result of diabetes, semistarvation, or insulin treatment. In human subjects, Na+-K+- ATPase concentration in vastus lateralis muscle biopsies was 17 and 22% greater (P < 0.05), respectively, in patients with treated non-insulin- dependent diabetes mellitus (n = 24) and insulin-dependent diabetes mellitus (n = 7) than in control subjects (n = 8). A positive linear correlation between muscle Na+-K+-ATPase and plasma insulin concentrations was observed (r = 0.50, P = 0.006; n = 29). Thus, insulin seems a regulator of muscle Na+-K+-ATPase concentration, reduction of muscle Na+-K+-ATPase concentration with untreated diabetes bears similarities with undernourishment, and physical conditioning may ameliorate the muscle Na+- K+-ATPase concentration decrease induced by diabetes.
AB - As determined by vanadate-facilitated [3H]ouabain binding to intact samples, semistarvation and untreated streptozotocin- or partial pancreatectomy-induced diabetes reduced rat soleus muscle Na+-K+- adenosinetriphosphatase (Na+-K+-ATPase) concentration by 12-21% (P < 0.05). Conversely, insulin treatment of rats with streptozotocin-induced diabetes induced an increase of 18-26% above control (P < 0.05). Treadmill training diminished the reduction in muscle [3H]ouabain binding site concentration induced by untreated diabetes to only 2-5%. No significant variation was observed in rat cerebral cortex Na+-K+-ATPase concentration as a result of diabetes, semistarvation, or insulin treatment. In human subjects, Na+-K+- ATPase concentration in vastus lateralis muscle biopsies was 17 and 22% greater (P < 0.05), respectively, in patients with treated non-insulin- dependent diabetes mellitus (n = 24) and insulin-dependent diabetes mellitus (n = 7) than in control subjects (n = 8). A positive linear correlation between muscle Na+-K+-ATPase and plasma insulin concentrations was observed (r = 0.50, P = 0.006; n = 29). Thus, insulin seems a regulator of muscle Na+-K+-ATPase concentration, reduction of muscle Na+-K+-ATPase concentration with untreated diabetes bears similarities with undernourishment, and physical conditioning may ameliorate the muscle Na+- K+-ATPase concentration decrease induced by diabetes.
KW - sodium-potassium adenosinetriphosphatase
KW - tritiated ouabain binding
KW - undernourishment
UR - http://www.scopus.com/inward/record.url?scp=0028322032&partnerID=8YFLogxK
U2 - 10.1152/jappl.1994.76.5.2140
DO - 10.1152/jappl.1994.76.5.2140
M3 - Article
C2 - 8063678
AN - SCOPUS:0028322032
VL - 76
SP - 2140
EP - 2146
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 5
ER -