TY - JOUR
T1 - Genotypic and phenotypic relatedness of 80 strains of Branhamella catarrhalis of worldwide origin
AU - Christensen, Jens Jørgen
AU - Ursing, Jan
AU - Bruun, Brita
PY - 1994/6/1
Y1 - 1994/6/1
N2 - 80 clinical Branhamella catarrhalis strains of worldwide origin were examined for genotypic relatedness and phenotypic characteristics. Using a quantitative bacterial dot method for DNA-DNA hybridization the strains were found to form a homogeneous group with ΔTm-values ranging from 0.0-2.3°C. In Minibact-N, an identification kit for oxidase positive, Gram-negative diplococci using eight phenotypic characteristics, all isolates were correctly identified and also demonstrated complete homogeneity except for β-lactamase production. Type strains representing the genera Branhamella, Moraxella and Neisseria were also examined for comparison. B. catarrhalis strain NCTC 4103-known to be atypical-had a ΔTm-value of 5.7°C and produced γ-glutamylaminopeptidase, in contrast to all other B. catarrhalis strains. In GN MicroPlateTM, a kit which tests utilizable carbon sources, B. catarrhalis strains were found to be able to utilize up to 16 to 95 carbon sources.
AB - 80 clinical Branhamella catarrhalis strains of worldwide origin were examined for genotypic relatedness and phenotypic characteristics. Using a quantitative bacterial dot method for DNA-DNA hybridization the strains were found to form a homogeneous group with ΔTm-values ranging from 0.0-2.3°C. In Minibact-N, an identification kit for oxidase positive, Gram-negative diplococci using eight phenotypic characteristics, all isolates were correctly identified and also demonstrated complete homogeneity except for β-lactamase production. Type strains representing the genera Branhamella, Moraxella and Neisseria were also examined for comparison. B. catarrhalis strain NCTC 4103-known to be atypical-had a ΔTm-value of 5.7°C and produced γ-glutamylaminopeptidase, in contrast to all other B. catarrhalis strains. In GN MicroPlateTM, a kit which tests utilizable carbon sources, B. catarrhalis strains were found to be able to utilize up to 16 to 95 carbon sources.
KW - Branhamella catarrhalis
KW - DNA-DNA hybridization
KW - GN MicroPlate
KW - Minibact-N
UR - http://www.scopus.com/inward/record.url?scp=0028238702&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.1994.tb06882.x
DO - 10.1111/j.1574-6968.1994.tb06882.x
M3 - Article
C2 - 8039655
AN - SCOPUS:0028238702
VL - 119
SP - 155
EP - 159
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 1-2
ER -