TY - JOUR
T1 - Effects of growth hormone administration on protein dynamics and substrate metabolism during 4 weeks of dietary restriction in obese women
AU - Nørrelund, Helene
AU - Børglum, Jens
AU - Jørgensen, Jens Otto Lunde
AU - Richelsen, Bjørn
AU - Møller, Niels
AU - Nair, K. Sreekumaran
AU - Christiansen, Jens Sandahl
PY - 2000/4/5
Y1 - 2000/4/5
N2 - OBJECTIVE: Treatment of obesity with very low calorie diet (VLCD) is complicated by protein loss. We evaluated the effects of coadministration of GH on protein turnover, substrate metabolism, and body composition in VLCD treated obesity. DESIGN AND PATIENTS: Fifteen obese women underwent 4 weeks of very low calorie diet (VLCD) in parallel with GH treatment (n = 7) or placebo (n = 8). MEASUREMENTS: Protein metabolism and total glucose turnover were isotopically assayed. Plasma concentrations of amino acids were determined by an HPLC system. Estimated rates of lipid and glucose oxidation were obtained by indirect calorimetry. Fat free mass was determined by DEXA- scan. RESULTS: Protein breakdown decreased in both groups (tyrosine flux μmol/h): -12% ± 3 (GH) vs. -9% ± 3 (placebo)). Phenylalanine degradation in relation to phenylalanine concentration decreased by 9% in the GH group, whereas an increase of 8% was observed in the placebo group (P = 0.1). Plasma concentrations of several amino acids were significantly decreased in the placebo group, while urea excretion decreased in the GH group. A decrease in FFM was found in placebo treated patients (2.14% ± 1.9 (GH) vs. -3.54% ± 1.6 (placebo), P < 0.05). Rates of lipid oxidation tended to be increased by GH treatment (lipid oxidation (mg/minutes): 79.7 ± 5.9 (GH) vs. 64.6 ± 5.9 (placebo), P = 0.1). CONCLUSION: During dietary restriction GH primarily seems to conserve protein by a reduced hepatic degradation of amino acids.
AB - OBJECTIVE: Treatment of obesity with very low calorie diet (VLCD) is complicated by protein loss. We evaluated the effects of coadministration of GH on protein turnover, substrate metabolism, and body composition in VLCD treated obesity. DESIGN AND PATIENTS: Fifteen obese women underwent 4 weeks of very low calorie diet (VLCD) in parallel with GH treatment (n = 7) or placebo (n = 8). MEASUREMENTS: Protein metabolism and total glucose turnover were isotopically assayed. Plasma concentrations of amino acids were determined by an HPLC system. Estimated rates of lipid and glucose oxidation were obtained by indirect calorimetry. Fat free mass was determined by DEXA- scan. RESULTS: Protein breakdown decreased in both groups (tyrosine flux μmol/h): -12% ± 3 (GH) vs. -9% ± 3 (placebo)). Phenylalanine degradation in relation to phenylalanine concentration decreased by 9% in the GH group, whereas an increase of 8% was observed in the placebo group (P = 0.1). Plasma concentrations of several amino acids were significantly decreased in the placebo group, while urea excretion decreased in the GH group. A decrease in FFM was found in placebo treated patients (2.14% ± 1.9 (GH) vs. -3.54% ± 1.6 (placebo), P < 0.05). Rates of lipid oxidation tended to be increased by GH treatment (lipid oxidation (mg/minutes): 79.7 ± 5.9 (GH) vs. 64.6 ± 5.9 (placebo), P = 0.1). CONCLUSION: During dietary restriction GH primarily seems to conserve protein by a reduced hepatic degradation of amino acids.
UR - http://www.scopus.com/inward/record.url?scp=0034105340&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2265.2000.00937.x
DO - 10.1046/j.1365-2265.2000.00937.x
M3 - Article
C2 - 10718828
AN - SCOPUS:0034105340
SN - 0300-0664
VL - 52
SP - 305
EP - 312
JO - Clinical Endocrinology
JF - Clinical Endocrinology
IS - 3
ER -