Diffusion-weighted MRI for verification of electroporation-based treatments

Faisal Mahmood, Rasmus H. Hansen, Birgit Agerholm-Larsen, Kurt S. Jensen, Helle K. Iversen, Julie Gehl

    Publikation: Bidrag til tidsskriftArtikelForskningpeer review

    Abstrakt

    Clinical electroporation (EP) is a rapidly advancing treatment modality that uses electric pulses to introduce drugs or genes into, e.g., cancer cells. The indication of successful EP is an instant plasma membrane permeabilization in the treated tissue. A noninvasive means of monitoring such a tissue reaction represents a great clinical benefit since, in case of target miss, retreatment can be performed immediately. We propose diffusion-weighted magnetic resonance imaging (DW-MRI) as a method to monitor EP tissue, using the concept of the apparent diffusion coefficient (ADC). We hypothesize that the plasma membrane permeabilization induced by EP changes the ADC, suggesting that DW-MRI constitutes a noninvasive and quick means of EP verification. In this study we performed in vivo EP in rat brains, followed by DW-MRI using a clinical MRI scanner. We found a pulse amplitude-dependent increase in the ADC following EP, indicating that (1) DW-MRI is sensitive to the EP-induced changes and (2) the observed changes in ADC are indeed due to the applied electric field.

    OriginalsprogEngelsk
    Sider (fra-til)131-138
    Antal sider8
    TidsskriftJournal of Membrane Biology
    Vol/bind240
    Udgave nummer3
    DOI
    StatusUdgivet - 1 apr. 2011

    Fingeraftryk Udforsk hvilke forskningsemner 'Diffusion-weighted MRI for verification of electroporation-based treatments' indeholder.

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