Deuterium MR spectroscopy at 4.7 T: Quantification of tumour and subcutaneous tissue blood flow in animal models

R. Wirestam*, V. Andrée Larsen, M. Stubgaard, C. Thomsen, B. Vikhoff, H. B.W. Larsson, F. Ståhlberg, O. Henriksen

*Corresponding author af dette arbejde

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    Abstrakt

    Deuterium MR spectroscopy was used for the determination of tissue blood flow (TBF). The tracer D2O was injected into the tissue of interest, and tracer washout was followed using a 4.7 T spectroscopy/imaging unit. Normal subcutaneous tissue in rats was studied, as well as tissue influenced by vasoactive agents (papaverine and adrenaline). The vasoactive agents introduced changes of 40% in TBF, compared with normal tissue. Normal tissue measurements were repeated using various D2O injection volumes (5–400 μl). The injection volume 5 μl gave TBF 11.7 ± 2.0 ml/100 g·min (mean ± 1 SD). This value was 40% higher than corresponding values observed at larger injection volumes (200–400 μl). This injection volume effect is probably partly due to a capillary dilution caused by tracer administration, and partly related to the non-physiological deuterium signal decrease observed in dead rats. Blood flow measurements in human colon tumours implanted in nude mice showed a rather poor reproducibility, not improved by the use of a multiple site injection technique.

    OriginalsprogEngelsk
    Sider (fra-til)85-91
    Antal sider7
    TidsskriftActa Radiologica
    Vol/bind36
    Udgave nummer1
    DOI
    StatusUdgivet - jan. 1995

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