Determination of pepsin (EC 3.4.23.1) and gastricsin (EC 3.4.23.3) in gastric juice by rocket immunoelectrophoresis

C. K. Axelsson*, N. H. Axelsen, P. B. Szecsi, B. Foltmann

*Corresponding author af dette arbejde

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    Abstract

    Antisera were raised in rabbits against chromatographically purified preparations of pepsin and gastricsin. With these antisera the contents of pepsin and gastricsin in gastric juice were determined by rocket immunoelectrophoresis. The potential content of pepsin and gastricsin of a secondary standard of gastric mucosal extract was calibrated against the chromatographically purified enzymes. This secondary standard was used for routine analyses. The intra-assay and between-assay precision was 3-4% and 6-9%, respectively. Ten healthy volunteers underwent a standard pentagastrin test. The amounts of pepsin and gastricsin determined by rocket immunoelectrophoresis corresponded to the amounts observed by ion exchange chromatography of gastric juice. After stimulation with pentagastrin the secretion of both pepsin and gastricsin was increased about 10 times.

    OriginalsprogEngelsk
    Sider (fra-til)323-331
    Antal sider9
    TidsskriftClinica Chimica Acta
    Vol/bind129
    Udgave nummer3
    DOI
    StatusUdgivet - 25 apr. 1983

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    Udforsk hvilke forskningsemner 'Determination of pepsin (EC 3.4.23.1) and gastricsin (EC 3.4.23.3) in gastric juice by rocket immunoelectrophoresis' indeholder.

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