CEA AND CARBOHYDRATE ANTIGENS IN NORMAL AND NEOPLASTIC COLON MUCOSA: An Immunohistochemical Study

Qin Ouyang, Mogens Viljen*, Birgitte Ravn Juhl, Lise Grupe Larsen, Vibeke Binder

*Corresponding author af dette arbejde

    Publikation: Bidrag til tidsskriftArtikelForskningpeer review

    Abstract

    Antibodies raised against tumour‐associated antigens have been assessed for tumour selectivity using an indirect immunohistochemical peroxidase staining of formalin‐fixed, paraffin‐embedded tissue from colon carcinomas, colon polyps and normal mucosa. The following antibodies were used: 1) Unabsorbed polyclonal antibody to carcinoembryonic antigen (poly‐CEA). 2) Monoclonal antibodies to CEA (mabs 3851 and 27). 3) Monoclonal antibodies to protein‐bound carbohydrates (mabs C 216 and C 242) or to lipid‐ and protein‐bound carbohydrates (C 50 and 19–9). These antibodies had been produced by hybridization of lymphocytes from mice, immunized with colon carcinoma cell lines or colon cancer tissue. All antibodies were used in one concentration only, preselected by initial titration experiments. No antibody was completely tumour‐specific, but four antibodies, mabs 3851, 27, C 216 and C 242, showed statistically significant tumour selectivity. Using these antibodies, respectively 19, 19, 19, and 18 of 20 colon cancers were stained compaired with 3, 4, 4, and 8 of 15 specimens of colon mucosa from normal controls. An increased frequency of staining was also noted in dysplastic polyps (statistically significant using mabs 3851 and C 216) and in dysplastic mucosa adjacent to a tumour (statistically significant using mabs 3851 and 27). The staining frequency of normal colon mucosa in cases of colon cancer did not differ from that in the normal controls. Poly‐CEA and the antiganglioside mabs C 50 and 19–9 revealed no tumour selectivity. A pronounced goblet cell staining was seen using C 50, C 242 and 19–9.

    OriginalsprogEngelsk
    Sider (fra-til)177-183
    Antal sider7
    TidsskriftActa Pathologica Microbiologica Scandinavica Series A :Pathology
    Vol/bind95 A
    Udgave nummer1-6
    DOI
    StatusUdgivet - jul. 1987

    Fingeraftryk

    Udforsk hvilke forskningsemner 'CEA AND CARBOHYDRATE ANTIGENS IN NORMAL AND NEOPLASTIC COLON MUCOSA: An Immunohistochemical Study' indeholder.

    Citationsformater