Biosynthetic capacity and regulatory aspects of prostaglandin E2 formation in adipocytes

Bjørn Richelsen, Jens D. Børglum, Søren S. Sørensen

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    Abstrakt

    In contrast to previous studies, Parker et al. (Diabetes (1989) 38, 1123) have recently found that isolated rat adipocytes alone were unable to synthesize prostaglandins (PG) and that the PG measured in adipocyte suspensions were due to contaminating non-adipocyte cells. In the present study the capacity of adipocytes to produce PGE2 has further been explored. Preparations of isolated rat adipocytes were extensively washed in order to get rid of contaminating cells. The released PGE2 was measured by radioimmunoassay (RIA) after high-performance liquid chromatography (HPLC) separation. We found that after repetive washing (up to 20 times) the isolated adipocytes were still able to synthesize PGE2 and this process was fully activatable by epinephrine, which indicates that pure adipocytes, themselves, are able to produce PGE2. However, addition of non-adipocyte material (from the adipose tissue) to 'pure' adipocytes (washed 10 times) enhanced the PGE2 synthesis significantly (P < 0.001) as compared to 'pure' adipocytes alone. Thus, some kind of synergy exists between adipocytes and non-adipocyte cells in the adipose tissue in respect to PG formation. Some regulatory aspects of PG synthesis in 'pure' adipocytes were also investigated. Phospholipase A2 (2 U/ml) enhanced PGE2 synthesis significantly (119 ± 21 to 658 ± 85 pg/106 cells, P < 0.001) without affecting lipolysis (glycerol release). The combined effect of epinephrine (5 μM) and phospholipase A2 (2 U/ml) on PGE2 formation was almost additive. Insulin inhibited the epinephrine-induced PG formation (P < 0.01) but had no effects on the action induced by phospholipase A2. In conclusion, there is no doubt that isolated adipocytes cooperate with non-adipocyte cells from the adipose tissue in respect to PG synthesis. Isolated adipocytes seem, however, still to have the ability to synthesize PGs, at least PGE2. Finally, both epinephrine and phospholipase A2 have the capacity for inducing PGE2 synthesis in isolated adipocytes.

    OriginalsprogEngelsk
    Sider (fra-til)73-81
    Antal sider9
    TidsskriftMolecular and Cellular Endocrinology
    Vol/bind85
    Udgave nummer1-2
    DOI
    StatusUdgivet - maj 1992

    Fingeraftryk Udforsk hvilke forskningsemner 'Biosynthetic capacity and regulatory aspects of prostaglandin E<sub>2</sub> formation in adipocytes' indeholder.

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