TY - JOUR
T1 - Assessing inter-rater reproducibility in MScanFit MUNE in a 6-subject, 12-rater "Round Robin" setup
AU - Sørensen, Daniel Mosgaard
AU - Bostock, Hugh
AU - Ballegaard, Martin
AU - Fuglsang-Frederiksen, Anders
AU - Graffe, Carolina Canillo
AU - Grötting, Arnstein
AU - Jones, Kelvin
AU - Kallio, Mika
AU - Krarup, Christian
AU - Krøigård, Thomas
AU - Lupescu, Tudor
AU - Maitland, Stuart
AU - Moldovan, Mihai
AU - Nilsen, Kristian Bernhard
AU - Pugdahl, Kirsten
AU - Santos, Miguel Oliveira
AU - Themistocleous, Andreas Constantinos
AU - Zlateva, Suzana Slavica
AU - Ööpik, Merle
AU - Tankisi, Hatice
N1 - Copyright © 2021 Elsevier Masson SAS. All rights reserved.
PY - 2022/4
Y1 - 2022/4
N2 - OBJECTIVE: To assess the inter-rater reliability of MScanFit MUNE using a "Round Robin" research design.METHODS: Twelve raters from different centres examined six healthy study participants over two days. Median, ulnar and common peroneal nerves were stimulated, and compound muscle action potential (CMAP)-scans were recorded from abductor pollicis brevis (APB), abductor digiti minimi (ADM) and anterior tibial (TA) muscles respectively. From this we calculated the Motor Unit Number Estimation (MUNE) and "A50", a motor unit size parameter. As statistical analysis we used the measures Limits of Agreement (LOA) and Coefficient of Variation (COV). Study participants scored their perception of pain from the examinations on a rating scale from 0 (no pain) to 10 (unbearable pain).RESULTS: Before this study, 41.6% of the raters had performed MScanFit less than five times. The mean MUNE-values were: 99.6 (APB), 131.4 (ADM) and 126.2 (TA), with LOA: 19.5 (APB), 29.8 (ADM) and 20.7 (TA), and COV: 13.4 (APB), 6.3 (ADM) and 5.6 (TA). MUNE-values correlated to CMAP max amplitudes (R2-values were: 0.463 (APB) (p<0.001), 0.421 (ADM) (p<0.001) and 0.645 (TA) (p<0.001)). The average perception of pain was 4.DISCUSSION: MScanFit indicates a high level of inter-rater reliability, even with only limited rater experience and is overall reasonably well tolerated by patients. These results may indicate MScanFit as a reliable MUNE method with potential as a biomarker in drug trials.
AB - OBJECTIVE: To assess the inter-rater reliability of MScanFit MUNE using a "Round Robin" research design.METHODS: Twelve raters from different centres examined six healthy study participants over two days. Median, ulnar and common peroneal nerves were stimulated, and compound muscle action potential (CMAP)-scans were recorded from abductor pollicis brevis (APB), abductor digiti minimi (ADM) and anterior tibial (TA) muscles respectively. From this we calculated the Motor Unit Number Estimation (MUNE) and "A50", a motor unit size parameter. As statistical analysis we used the measures Limits of Agreement (LOA) and Coefficient of Variation (COV). Study participants scored their perception of pain from the examinations on a rating scale from 0 (no pain) to 10 (unbearable pain).RESULTS: Before this study, 41.6% of the raters had performed MScanFit less than five times. The mean MUNE-values were: 99.6 (APB), 131.4 (ADM) and 126.2 (TA), with LOA: 19.5 (APB), 29.8 (ADM) and 20.7 (TA), and COV: 13.4 (APB), 6.3 (ADM) and 5.6 (TA). MUNE-values correlated to CMAP max amplitudes (R2-values were: 0.463 (APB) (p<0.001), 0.421 (ADM) (p<0.001) and 0.645 (TA) (p<0.001)). The average perception of pain was 4.DISCUSSION: MScanFit indicates a high level of inter-rater reliability, even with only limited rater experience and is overall reasonably well tolerated by patients. These results may indicate MScanFit as a reliable MUNE method with potential as a biomarker in drug trials.
KW - Action Potentials/physiology
KW - Amyotrophic Lateral Sclerosis
KW - Electromyography/methods
KW - Humans
KW - Motor Neurons/physiology
KW - Muscle, Skeletal/innervation
KW - Pain
KW - Reproducibility of Results
U2 - 10.1016/j.neucli.2021.11.002
DO - 10.1016/j.neucli.2021.11.002
M3 - Article
C2 - 34906430
SN - 0987-7053
VL - 52
SP - 157
EP - 169
JO - Neurophysiologie Clinique
JF - Neurophysiologie Clinique
IS - 2
ER -