An assessment of ADAMs in bone cells: absence of TACE activity prevents osteoclast recruitment and the formation of the marrow cavity in developing long bones

Patrice Boissy, Thomas R Lenhard, Tove Kirkegaard, Jacques J Peschon, Roy A Black, Jean-Marie Delaissé, Maria del Carmen Ovejero

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    Abstrakt

    ADAMs (A Disintegrin And Metalloprotease domain) are metalloprotease-disintegrin proteins that have been implicated in cell adhesion, protein ectodomain shedding, matrix protein degradation and cell fusion. Since such events are critical for bone resorption and osteoclast recruitment, we investigated whether they require ADAMs. We report here which ADAMs we have identified in bone cells, as well as our analysis of the generation, migration and resorptive activity of osteoclasts in developing metatarsals of mouse embryos lacking catalytically active ADAM 17 [TNFalpha converting enzyme (TACE)]. The absence of TACE activity still allowed the generation of cells showing an osteoclastic phenotype, but prevented their migration into the core of the diaphysis and the subsequent formation of marrow cavity. This suggests a role of TACE in the recruitment of osteoclasts to future resorption sites.

    OriginalsprogEngelsk
    Sider (fra-til)257-61
    Antal sider5
    TidsskriftFEBS Letters
    Vol/bind553
    Udgave nummer3
    DOI
    StatusUdgivet - 23 okt. 2003

    Fingeraftryk Udforsk hvilke forskningsemner 'An assessment of ADAMs in bone cells: absence of TACE activity prevents osteoclast recruitment and the formation of the marrow cavity in developing long bones' indeholder.

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