TY - JOUR
T1 - Aerococcus urinae
T2 - Polyphasic characterization of the species
AU - Christensen, Jens Jørgen
AU - Kilian, M.
AU - Fussing, V.
AU - Andresen, K.
AU - Blom, J.
AU - Korner, B.
AU - Steigerwalt, A. G.
PY - 2005/7/1
Y1 - 2005/7/1
N2 - A polyphasic characterization of Aerococcus urinae is presented. In this study the intraspecies relationships between 26 strains of varying geographical origin were examined by phenotypic tests, ribotyping and multilocus enzyme electrophoresis. The results demonstrated two main phenotypic patterns that could be distinguished in tests for hydrolysis of aesculin, and acid production from amygdalin and salicin. Strains were either negative (n = 19) or positive (n = 6) in these tests. One strain had a deviating pattern. Heterogeneity within the 19 pattern I strains was demonstrated especially by phenotypic tests (acid production from ribose, mannitol, sorbitol, sucrose and D-arabitol) and by multilocus enzyme electrophoresis. However, DNA sequence analysis of the 16S rRNA (n = 7) and gyrB genes (n = 3) from strains representing the two main patterns showed no variation in sequences among strains. Comparison of A. urinae and representatives of related taxa by 16S rDNA sequence analysis showed that the taxon is related to, but distinct from, other Aerococcus spp.
AB - A polyphasic characterization of Aerococcus urinae is presented. In this study the intraspecies relationships between 26 strains of varying geographical origin were examined by phenotypic tests, ribotyping and multilocus enzyme electrophoresis. The results demonstrated two main phenotypic patterns that could be distinguished in tests for hydrolysis of aesculin, and acid production from amygdalin and salicin. Strains were either negative (n = 19) or positive (n = 6) in these tests. One strain had a deviating pattern. Heterogeneity within the 19 pattern I strains was demonstrated especially by phenotypic tests (acid production from ribose, mannitol, sorbitol, sucrose and D-arabitol) and by multilocus enzyme electrophoresis. However, DNA sequence analysis of the 16S rRNA (n = 7) and gyrB genes (n = 3) from strains representing the two main patterns showed no variation in sequences among strains. Comparison of A. urinae and representatives of related taxa by 16S rDNA sequence analysis showed that the taxon is related to, but distinct from, other Aerococcus spp.
KW - Aerococcus urinae
KW - DNA sequence analysis
KW - Multilocus enzyme electrophoresis
KW - Phenotypic tests
KW - Ribotyping
KW - Transmission electron microscopy
UR - http://www.scopus.com/inward/record.url?scp=23144446589&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0463.2005.apm_183.x
DO - 10.1111/j.1600-0463.2005.apm_183.x
M3 - Article
C2 - 16086822
AN - SCOPUS:23144446589
SN - 0903-4641
VL - 113
SP - 517
EP - 525
JO - APMIS
JF - APMIS
IS - 7-8
ER -