A simple and rapid method for the determination of the number of 3H-ouabain binding sites in biopsies of skeletal muscle

Aage NØrgaard*, Keld Kjeldsen, Otto Hansen, Torben Clausen

*Corresponding author af dette arbejde

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    Abstract

    Based on vanadate facilitation of 3H-ouabain binding to Na-K-ATPase, a method has been developed allowing the measurement of the number of 3H-ouabain binding sites in small rat muscle biopsies (2-14 mg). Comparison with results obtained using intact fibres showed close agreement over the wide range of values for 3H-ouabain binding sites (100-900 pmol per g wet weight) associated with variations in age, thyroid status and K-deficiency.

    OriginalsprogEngelsk
    Sider (fra-til)319-325
    Antal sider7
    TidsskriftBiochemical and Biophysical Research Communications
    Vol/bind111
    Udgave nummer1
    DOI
    StatusUdgivet - 28 feb. 1983

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