A New Tool for Analyses of Whole Genome Sequences Reveals Dissemination of Specific Strains of Vancomycin-Resistant Enterococcus faecium in a Hospital

Lis Marbjerg; Caroline Louise Stougaard; Sophie-Amalie Grønhøj Sørensen; Amalie Vørs Thomsen; Lis Wang; Lise Andersen; Thomas Emil Andersen; Birgitte Kallipolitis; Michael Kemp

doi:10.3389/fmed.2021.733676

A New Tool for Analyses of Whole Genome Sequences Reveals Dissemination of Specific Strains of Vancomycin-Resistant Enterococcus faecium in a Hospital

Lis Marbjerg, Caroline Louise Stougaard, Sophie-Amalie Grønhøj Sørensen, Amalie Vørs Thomsen, Lis Wang, Lise Andersen, Thomas Emil Andersen, Birgitte Kallipolitis, Michael Kemp^*

^*Corresponding author af dette arbejde

Klinisk Mikrobiologisk Afdeling

Publikation: Bidrag til tidsskrift › Artikel › Forskning › peer review

Abstract

A new easy-to-use online bioinformatic tool analyzing whole genome sequences of healthcare associated bacteria was used by a local infection control unit to retrospectively map genetic relationship of isolates of E. faecium carrying resistance genes to vancomycin in a hospital. Three clusters of isolates were detected over a period of 5 years, suggesting transmission between patients. Individual relatedness between isolates within each cluster was established by SNP analyses provided by the system. Genetic antimicrobial resistance mechanisms to antibiotics other than vancomycin were identified. The results suggest that the system is suited for hospital surveillance of E. faecium carrying resistance genes to vancomycin in settings with access to next Generation Sequencing without bioinformatic expertise for interpretation of the genome sequences.

Originalsprog	Engelsk
Artikelnummer	733676
Tidsskrift	Frontiers in Medicine
Vol/bind	8
DOI	https://doi.org/10.3389/fmed.2021.733676
Status	Udgivet - 27 okt. 2021

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10.3389/fmed.2021.733676

Citationsformater

Marbjerg, L., Stougaard, C. L., Sørensen, S.-A. G., Thomsen, A. V., Wang, L., Andersen, L., Andersen, T. E., Kallipolitis, B., & Kemp, M. (2021). A New Tool for Analyses of Whole Genome Sequences Reveals Dissemination of Specific Strains of Vancomycin-Resistant Enterococcus faecium in a Hospital. Frontiers in Medicine, 8, Artikel 733676. https://doi.org/10.3389/fmed.2021.733676

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title = "A New Tool for Analyses of Whole Genome Sequences Reveals Dissemination of Specific Strains of Vancomycin-Resistant Enterococcus faecium in a Hospital",

abstract = "A new easy-to-use online bioinformatic tool analyzing whole genome sequences of healthcare associated bacteria was used by a local infection control unit to retrospectively map genetic relationship of isolates of E. faecium carrying resistance genes to vancomycin in a hospital. Three clusters of isolates were detected over a period of 5 years, suggesting transmission between patients. Individual relatedness between isolates within each cluster was established by SNP analyses provided by the system. Genetic antimicrobial resistance mechanisms to antibiotics other than vancomycin were identified. The results suggest that the system is suited for hospital surveillance of E. faecium carrying resistance genes to vancomycin in settings with access to next Generation Sequencing without bioinformatic expertise for interpretation of the genome sequences.",

keywords = "vancomycin resistant enterococci, whole genome sequencing, bioinformatics, genomic epidemiology, infection control, surveillance",

author = "Lis Marbjerg and Stougaard, {Caroline Louise} and S{\o}rensen, {Sophie-Amalie Gr{\o}nh{\o}j} and Thomsen, {Amalie V{\o}rs} and Lis Wang and Lise Andersen and Andersen, {Thomas Emil} and Birgitte Kallipolitis and Michael Kemp",

note = "Copyright {\textcopyright} 2021 Marbjerg, Stougaard, S{\o}rensen, Thomsen, Wang, Andersen, Andersen, Kallipolitis and Kemp.",

year = "2021",

month = oct,

day = "27",

doi = "10.3389/fmed.2021.733676",

language = "English",

volume = "8",

journal = "Frontiers in Medicine",

issn = "2296-858X",

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TY - JOUR

T1 - A New Tool for Analyses of Whole Genome Sequences Reveals Dissemination of Specific Strains of Vancomycin-Resistant Enterococcus faecium in a Hospital

AU - Marbjerg, Lis

AU - Stougaard, Caroline Louise

AU - Sørensen, Sophie-Amalie Grønhøj

AU - Thomsen, Amalie Vørs

AU - Wang, Lis

AU - Andersen, Lise

AU - Andersen, Thomas Emil

AU - Kallipolitis, Birgitte

AU - Kemp, Michael

PY - 2021/10/27

Y1 - 2021/10/27

N2 - A new easy-to-use online bioinformatic tool analyzing whole genome sequences of healthcare associated bacteria was used by a local infection control unit to retrospectively map genetic relationship of isolates of E. faecium carrying resistance genes to vancomycin in a hospital. Three clusters of isolates were detected over a period of 5 years, suggesting transmission between patients. Individual relatedness between isolates within each cluster was established by SNP analyses provided by the system. Genetic antimicrobial resistance mechanisms to antibiotics other than vancomycin were identified. The results suggest that the system is suited for hospital surveillance of E. faecium carrying resistance genes to vancomycin in settings with access to next Generation Sequencing without bioinformatic expertise for interpretation of the genome sequences.

AB - A new easy-to-use online bioinformatic tool analyzing whole genome sequences of healthcare associated bacteria was used by a local infection control unit to retrospectively map genetic relationship of isolates of E. faecium carrying resistance genes to vancomycin in a hospital. Three clusters of isolates were detected over a period of 5 years, suggesting transmission between patients. Individual relatedness between isolates within each cluster was established by SNP analyses provided by the system. Genetic antimicrobial resistance mechanisms to antibiotics other than vancomycin were identified. The results suggest that the system is suited for hospital surveillance of E. faecium carrying resistance genes to vancomycin in settings with access to next Generation Sequencing without bioinformatic expertise for interpretation of the genome sequences.

KW - vancomycin resistant enterococci

KW - whole genome sequencing

KW - bioinformatics

KW - genomic epidemiology

KW - infection control

KW - surveillance

U2 - 10.3389/fmed.2021.733676

DO - 10.3389/fmed.2021.733676

M3 - Article

C2 - 34778297

SN - 2296-858X

VL - 8

JO - Frontiers in Medicine

JF - Frontiers in Medicine

M1 - 733676

ER -

A New Tool for Analyses of Whole Genome Sequences Reveals Dissemination of Specific Strains of Vancomycin-Resistant Enterococcus faecium in a Hospital

Abstract

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