A flow-through microsampling device applied to an ion-exchange chromatography system

Johan Nilsson; Pal Szeczi; Claus Schafer-Nielsen

doi:10.1016/0165-022X(93)90002-6

A flow-through microsampling device applied to an ion-exchange chromatography system

Johan Nilsson^*
, Pal Szeczi
, Claus Schafer-Nielsen

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift › Artikel › Forskning › peer review

Abstract

A flow-through microsampling device that can be inserted in the column elution flow line of a chromatographic system has been developed. A sample drop (volume 2.2 nl) is ejected when a voltage pulse is applied to a piezo-electric transducer. The drops can be ejected with up to 70 Hz frequency, giving a sample flow range of 0-9 μl/min. Samples were collected on a moving agarose surface during elution of freshly prepared human serum. The precipitate pattern obtained after immunoelectrophoresis is similar to what would be obtained in a fused rocket electrophoresis of multiple samples from a fraction collector.

Originalsprog	Engelsk
Sider (fra-til)	181-190
Antal sider	10
Tidsskrift	Journal of Biochemical and Biophysical Methods
Vol/bind	27
Udgave nummer	3
DOI	https://doi.org/10.1016/0165-022X(93)90002-6
Status	Udgivet - okt. 1993

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10.1016/0165-022X(93)90002-6

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abstract = "A flow-through microsampling device that can be inserted in the column elution flow line of a chromatographic system has been developed. A sample drop (volume 2.2 nl) is ejected when a voltage pulse is applied to a piezo-electric transducer. The drops can be ejected with up to 70 Hz frequency, giving a sample flow range of 0-9 μl/min. Samples were collected on a moving agarose surface during elution of freshly prepared human serum. The precipitate pattern obtained after immunoelectrophoresis is similar to what would be obtained in a fused rocket electrophoresis of multiple samples from a fraction collector.",

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author = "Johan Nilsson and Pal Szeczi and Claus Schafer-Nielsen",

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AU - Szeczi, Pal

AU - Schafer-Nielsen, Claus

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N2 - A flow-through microsampling device that can be inserted in the column elution flow line of a chromatographic system has been developed. A sample drop (volume 2.2 nl) is ejected when a voltage pulse is applied to a piezo-electric transducer. The drops can be ejected with up to 70 Hz frequency, giving a sample flow range of 0-9 μl/min. Samples were collected on a moving agarose surface during elution of freshly prepared human serum. The precipitate pattern obtained after immunoelectrophoresis is similar to what would be obtained in a fused rocket electrophoresis of multiple samples from a fraction collector.

AB - A flow-through microsampling device that can be inserted in the column elution flow line of a chromatographic system has been developed. A sample drop (volume 2.2 nl) is ejected when a voltage pulse is applied to a piezo-electric transducer. The drops can be ejected with up to 70 Hz frequency, giving a sample flow range of 0-9 μl/min. Samples were collected on a moving agarose surface during elution of freshly prepared human serum. The precipitate pattern obtained after immunoelectrophoresis is similar to what would be obtained in a fused rocket electrophoresis of multiple samples from a fraction collector.

KW - Flow-through liquid sampler

KW - Fused rocket electrophoresis

KW - Piezo-electric drop generator

UR - https://www.scopus.com/pages/publications/0027451905

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JO - Journal of Biochemical and Biophysical Methods

JF - Journal of Biochemical and Biophysical Methods

IS - 3

ER -

A flow-through microsampling device applied to an ion-exchange chromatography system

Abstract

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